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Ozone Therapy 101 In Holistic Medicine

Ozone can kill Lyme disease, cancer, and candida upon contact. Ozone therapy also improves cellular regeneration and many other medical conditions. There are many ways of administering ozone for health reasons, so here is a complete guide to ozone therapy in medicine: What is ozone therapy? Is IV ozone safe? How does the hyperbaric 10-pass ozone work? What do ozone injections do for your body?

What is Ozone Therapy?

(O3) Ozone molecule is made of 3 oxygen molecules with an electrical current and used for ozone therapy at our alternative medicine center in Mexico
Ozone Therapy is an alternative medical procedure that uses O3 administered to a patient to neutralize bacteria, viruses, fungi, yeast, and protozoa. Ozone also activates the immune system and reduces inflammation caused by disease.

What is Ozone (O3)? – The Chemical Compound

Ozone is a gas made up of 3 oxygen atoms fused by an electrical current. Ozone has no color so it is invisible to the eyes, but its effects are all around us. There is a protective layer of ozone gas surrounding our planet that keeps harmful UV rays out and disintegrates meteors so they do not enter our atmosphere. Ozone therapy has a similar effect on the body; it helps keep out bad things and disintegrates foreign agents.

At Holistic Bio Spa™– Stem Cell Therapy, we have always been invested in the health of our patients and we have been using ozone therapy to eliminate Lyme disease and cancer for over 10 years. Now with the acquisition of the 10-Pass Hyperbaric Ozone Generator, we particularly use this impressive device to deliver 10x more ozone safely in a single session for Lyme disease, cancer, and other serious illnesses.

In 2019, Holistic Bio Spa™ became the first medical center to have the 10 pass hyperbaric ozone generator in all Latin America.

What Medical Conditions Does Ozone Therapy Treat?

For over 150 years, ozone therapy has been used and heavily studied in medicine. The effects of ozone in the body are safe, consistent, and proven with very minimal and preventable side effects. Today, medical ozone is used successfully against
Lyme disease, cancer, and degenerative disorders. Ozone must be administered by a qualified doctor or naturopathic practitioner (Reference: International Ozone Association).

Ozone treatments are effective for almost every medical condition resulting from a pathogen, inflammation, and/or a suppressed immune system. We use ozone for the following:

A doctor injecting ozone into a patient's knee at Holistic Bio Spa - Stem Cell Therapy in Mexico
  • Ancillary health benefits of ozone therapy:
    • Increases energy
    • Reduces acidity
    • Makes nails and hair grow longer and stronger
    • When used in a gas chamber, adds a healthy glow to hair
    • Makes sleeping easier
    • Normalizes hormone and enzyme production
    • Reduces cardiac arrhythmia
    • Reduces diabetes complications
    • improving brain function and memory
    • Stops abnormal bleeding
    • Reduces pain
    • Facilitates mesenchymal stem cell production
Collage of patients getting alternative medical treatments from our medical team in Puerto Vallarta, Mexico

How is Ozone Administered in Medicine?

Our qualified physicians and doctors administer ozone in gaseous form at our stem cell therapy and alternative medicine center in Mexico using the following methods:
(IV) Intravenous Ozone Therapy
  • 10 pass hyperbaric ozone (2 hours+)
  • Major autohemotherapy (MAH) (30-45 minutes)
  • Ozone-infused saline solution (per the Madrid Convention’s protocol) (30-45 minutes)

Ozone soft tissue/joint injection
  • Intramuscular and intra-articular injection (15 minutes)
  • Prolozone injection (15 minutes)

Topical & Insufflation Ozone
  • Rectal insufflation (15 minutes)
  • Vaginal insufflation (15 minutes)
  • Oral/Dental rinse (15 minutes)
  • Body spray (5 minutes)

Method 1 - Ten-Pass Hyperbaric Ozone Therapy

Dr. Zoli receiving ten pass hyperbaric ozone therapy at Holistic Bio Spa
The 10-pass hyperbaric ozone therapy safely delivers a powerful dose of pure ozone to a patient’s bloodstream in under 2 hours. This advanced ozone generator combines positive (hyperbaric) and negative (vacuum) pressure with a closed-circuit or closed-loop autohemotherapy system to:


  1. Withdraw 200 cc of blood slowly by vacuum
  2. Heparinize the blood so it doesn’t clot
  3. Mix blood with 200cc of O2 and O3 mixture at 70 Mcg/ml
  4. Return ozonated blood into the bloodstream under 2 ATA pressure
  5. Repeat for 10 passes
  • Procedure duration

    How long is the 10-Pass Hyperbaric Ozone Therapy?

    Ten passes, or ten times the treatment of a single pass, can be given within 60-90 minutes by using a special ozone machine to vacuum the blood into a glass bottle, then return it rapidly by applying pressure into the bottle.

  • Superiority of method

    Is the 10-Pass Ozone better than regular IV ozone therapy?

    Yes. Using a 10-pass method instead of the traditional MAH results in more ozone concentrations in a shorter length of time and penetrate deeper saturation of erythrocytes with oxygen. Also, the positive pressure during hyperbaric ozone applications protects the red blood cells from being damaged when mixing/swirling the blood in the bottle.

  • Safety and side effects

    Is the 10-Pass Hyperbaric Ozone Treatment safe?

    Yes. From its inception, the10-pass has been studied and proven safe. Since it is a very powerful treatment, depending on the patient’s condition, the 10-pass can be used from 1 to 6 times a week. Anecdotal reports from patients and practitioners indicate that patients with Lyme Disease that have gone into remission from high dose ozone therapy have needed anywhere from about 10-30 treatments. Ozone is toxic if inhaled, so only a qualified physician should administer ozone for medicinal purposes.

    Side-effects are minimal and may last a couple of hours including feeling tired and sleepy before feeling energized.

  • 10x the ozone

    Why is it called 10-pass?

    “Ten-pass” means that this method enables us to safely give you, the patient, 10x the ozone of a single traditional pass. The entire ozone treatment is completed within 60-90 minutes making the blood ultra-clean, boosting the immune system, and eliminating bacteria, viruses, fungi, yeast, and protozoa.

  • Origin of method

    Where did the 10-pass ozone originate from?

    The method was invented by Dr. Johann Lahondy, an Austrian Gynecologist and surgeon who was retired and started experimenting on himself to gauge the effects of the treatment.

    For all of you referring international doctors and health enthusiasts reading this, here’s the general idea of how Dr. Lahondy came upon this discovery:

    • 200 ml of blood
    • 200 ml of O3/O2 ozone gas at 70 mcg/ml
    • Approx. 6,500 to 12,000 IU of Heparin
    • The blood is mixed and re-infused at 0.8 hyperbaric pressure
    • He repeated this 10 times within 1 to 2 hours

    Discovery: the blood has to be drawn and treated 10 times = this is where we get the name, “10-pass”.

    The traditional MAH (Major Autohemotherapy) ozone uses this standard:

    • 100 ml of O3/O2 at 30 Mcg/ml
    • 3000 mcg of ozone

    Regular ozone IV uses only 3,000 Mcg/ml of ozone. This means that ten-pass ozone supplies nearly 50 times the amount of ozone of a regular intravenous ozone treatment.

    The current tested protocol for the 10-pass ozone for each pass includes a total concentration of 140,000 Mcg/ml of O3/O2.

    • 200 ml of O3/O2 at 70 Mcg/ml
    • This protocol multiplied by 10 passes = 2,000 ml volume of O3/O2.
  • Does the 10-pass work for Lyme disease?
    Yes. To confirm the superiority of this method, many Lyme disease patients have cured themselves with just a single session of 10-pass ozone. For select Lyme disease patients, we offer a free online consultation with one of our doctors. Send us a message!
  • Can the 10-pass improve Stem Cell Therapy Results?
    Yes, because the 10-pass hyperbaric ozone therapy uses high concentrations of ozone that facilitates mesenchymal stem cell production, therefore aiding your body to produce new healthy cells faster and in higher quantities.
  • Can the 10-pass help with Long COVID symptoms?

    Yes the 10-pass can help with Long COVID symptoms because the 10-pass hyperbaric ozone therapy neutralizes bacteria, viruses, fungi, yeast, and protozoa. Ozone also activates the immune system and reduces inflammation caused by disease.

Patient Reviews for Ozone Therapy at Holistic Bio Spa

As a frequent client of Holistic Bio Spa, I had many of their awesome services over the last 5 years like SCIO Quantum Therapy; a very good, fast recovery with Ozone Therapy; and today finally Dr. Zoli and his very professional team did a complete Stem Cell Therapy. I do highly recommend to anyone who wants to heal fast and turn back the clock of aging.

Lazlo G.- patient review of ozone therapy on Google

It was the unusual and the best detox experience, starting with colon hydrotherapy therapy (out of this world, I could not believe I was so toxic). The exercise with oxygen was an amazing therapy full of benefits. The technology used for PRP, ozone facial, liposuction without surgery are wonderful!

Jess- patient review of Holistic Bio Spa on Google

Method 2 - Ozone Major Autohemotherapy

Without the use of the 10-pass ozone generator, the MAH ozone protocol involves the withdrawal of the patient’s blood. The blood is infused with ozone that produces oxidized compounds. The treated blood is then reinfused into the patient (in a closed circuit if desired).

Method 3 - Intravenous Ozone

An ozone-concentrated solution is delivered to the bloodstream via IV drip. The ozone is safely carried by the red blood cells all over the body with instant healing benefits that kill Lyme disease, cancer, bacterial infections, fungi, yeast, and protozoa upon contact.

Method 4 - Rectal Ozone

Ozone in gas form is gently administered via the rectum to target major organs such as the colon. From the rectum, the ozone enters the bloodstream fast, applying the healing benefits at a larger scale. While very effective, only a few CC of gas can enter the colon at a time, so this method requires multiple sessions.

Method 5 - Vaginal Ozone

Similar to rectal ozone, this procedure gently delivers pure ozone in gas form to the vaginal cavity. From the vagina, the ozone spreads to clear up the genital region and enters the bloodstream killing foreign bacteria and pathogens upon contact. This method has proven successful many times for curing HPV and other sexually transmitted diseases.

While very effective, only a few CC of gas can enter the vagina at a time, so this method requires multiple sessions. The same principle can apply for men, administering the ozone via the penis’ urethra, but it is not a “class favorite” as you can imagine. Pregnant women or women who have an IUD should not receive this form of ozone. Other options should be reviewed with the patient.

Method 6 - Dental/Oral Ozone

Ozone should not be inhaled, so insufflations are not to be done in the oral cavity. But ozone-infused water and other solutions can be prepared to rinse infected gum tissues (periodontal disease) and applied directly to cavities to kill harmful bacteria and reset pH levels in the mouth.

Method 7 - Prolozone Injections

Prolozone is an ozonated solution mixed with anti-inflammatory medications, procaine, homeopathic medicine, minerals, vitamins, etc. to a degenerated or injured joints to ease pain, facilitate regeneration, and improve the results of stem cell therapy.

This is likewise useful to regenerate and strengthen any joint, tendon, muscle, or skin tissue in the body.

Method 8 - Intra-articular & Intramuscular Ozone Injections

A direct injection of pure ozone in a join or soft tissue can eliminate pathogens causing arthritis, reduce inflammation, increase tissue regeneration, and help heal sports injuries or degeneration of tendon, ligaments, and cartilage. This is useful for wrists, elbows, shoulder, knee, spinal cord, and anit-aging solutions.

Method 9 - Topical Ozone Spray

We have a special ozonated liquid that can be sprayed on wounds, bruises, acne breakouts, and aging skin to repair damage and immediately disinfect the region.

Ready to Get Ozone Therapy at Holistic Bio Spa?

Our experts at the Holistic Bio Spa will use ozone therapy and complementing holistic treatments to help you heal from Lyme disease, cancer, and other serious illnesses. Ask any questions you have about ozone therapy or book your next appointment at our health center in tropical Puerto Vallarta or Nuevo Vallarta, Mexico.

Some of the Locations Our Healed Patients Visit From:

  • United States
  • Canada
  • Europe
  • Central America
  • Puerto Vallarta
  • Bucerias
  • Marina Vallarta
  • Nuevo Vallarta
  • Mismaloya
  • Amapas